动物造模 z-bio 2022-07-12 692

　　Objective: To screen the pathogenic related genes of enterovirus 71 through genetically diverse mouse resources, to deeply understand the pathogenic mechanism of the virus, and to provide the basis for clinical diagnosis, treatment and prognosis.

　　METHODS: 107copies EV71 virus was intraperitoneally infected with genetically diverse mouse strains aged 4-6 weeks, blood and hindlimb skeletal muscle samples were collected, and the viral load was analyzed by real-time quantitative PCR technology, and then the infection data was analyzed by The GeneMine phylogenetic database. , and screened to obtain EV71 pathogenic related genes.

　　RESULTS: Fifty-three genes related to EV71 pathogenicity were screened by genetically diverse mouse infection data.

　　Conclusion: Genetically diverse mice have great advantages in simulating population genetic diversity and studying diseases with complex traits, and are an ideal new animal resource for screening disease-related genes.