动物造模 z-bio 2022-09-30 549

　　OBJECTIVE: To explore the effect of myocardial tissue-specific Isca1 knockout on rat heart structure by magnetic resonance analysis and pathological analysis of myocardial tissue-specific Isca1 knockout rats.

　　Methods: The myocardial tissue-specific Isca1 knockout rats were bred, and the genotype and gene knockout efficiency of the rats were identified by PCR. The 0.5d and 2.5d neonatal myocardial tissue-specific Isca1 knockout rats were analyzed by MRI. The myocardial tissue of newborn 2.5-day-old myocardial tissue-specific Isca1-knockout rats was subjected to H&E staining and transmission electron microscopy analysis.

　　RESULTS: The knockout efficiency of myocardial tissue-specific Isca1 knockout rats was greater than 78%; compared with wild-type, myocardial tissue-specific Isca1 knockout rats showed no significant dilatation at 0.5 days; myocardial tissue-specific Isca1 knockout at 2.5 days The right ventricle of the rat heart showed a tendency to expand; 2.5d myocardial tissue-specific Isca1 knockout rat muscle fibers were irregularly arranged, with disordered arrangement, no hierarchy or polar orientation, some myocardial fibers were dissolved and broken, sarcomeres and Z lines were blurred, and The sarcolemma is damaged, the mitochondrial cristae are broken, and swelling is obvious.

　　Conclusion: Myocardial tissue-specific Isca1 knockout causes abnormal cardiac structure in neonatal rats.