动物造模,亚型犬流感病毒 z-bio 2022-09-30 351

　　Objective: To determine the type and distribution of influenza virus receptors in different tissues and organs of dogs, and to verify the susceptibility of dogs to H3N2 subtype canine influenza virus.

　　Methods: Different tissues and organs of healthy English Springer spaniels were collected, and the lectin immunofluorescence tissue staining method was used to detect the human influenza virus sialic acid receptor sialic acid α2,6-galactose (SAα2,6Gal) and the avian type in the tissue. Types and distribution of sialic acid receptor sialic acid α2,3-galactose (SAα2, 3Gal) of influenza virus; English Springer spaniels were artificially infected with H3N2 subtype canine influenza virus, and different tissues and organs were collected and analyzed by immunohistochemical staining Differential analysis of the tissue distribution of the virus.

　　Results: There are two types of receptors, SAα2,6Gal and SAα2,3Gal, in many tissues and organs, and a single receptor exists in a few tissues and organs; SAα-2,6Gal receptors are widely distributed and in the same organ The expression level of the virus is higher than that of the SAα-2,3Gal receptor. After artificial infection, the distribution of the virus is basically the same as the overall trend of the receptor amount, and there are only differences in individual organs.

　　Conclusion: Avian and human influenza virus receptors are widely distributed in various tissues and organs of dogs, and dogs have the molecular basis for simultaneous infection of avian and human influenza viruses.