动物造模 z-bio 2022-10-17 425

　　OBJECTIVE: To screen for microsatellite loci suitable for the detection of cat genetic quality.

　　Methods: 74 microsatellite loci were selected from relevant literature and GenBank, and the optimal annealing temperature was explored to optimize the PCR reaction conditions. Based on the results of agarose gel electrophoresis and STR scanning, clear and stable bands with allelic A set of microsatellite loci, which are gene-rich and evenly distributed on cat 18 pairs of autosomes, were used to detect the genetic quality of experimental cats.

　　Results: From 74 candidate microsatellite loci, 55 STR loci with polymorphism and stable amplification were screened. And 1~2 STR loci were appropriately selected in each autosomal chromosome (except A1 and B1 chromosomes) of cats. Finally, 31 microsatellite loci distributed in 18 pairs of autosomes with many alleles were obtained.

　　Conclusion: A set of microsatellite loci suitable for the analysis of the genetic structure of the cat population was successfully screened.