动物造模 z-bio 2022-11-11 414

　　Objective: To screen out the single nucleotide polymorphisms that can rapidly identify four commonly used rat strains [Wistar rat, Goto-Kakizaki rat, Brown-Norway (BN) rat, Sprague-Dawley (SD) rat] sex locus and verify its reliability.

　　Methods: First, whole genome resequencing of Wistar rats and GK rats was performed, and the resequencing results were compared with the reference genome of BN rats by using bioinformatics technology, and the specific genome-wide Wistar rats and GK rats were screened. A set of candidate SNP loci were further screened out, and the above candidate SNP loci were further screened and verified in Wistar, GK, BN and SD rat populations by next-generation sequencing and DNA mixed pooling method.

　　Results: Wistar and GK rats obtained 94,800 and 106,019 homozygous specific SNPs, respectively, among which 56,216 SNPs were shared by Wistar and GK rats. There are only 38 loci with different genotypes among the three rats, and 15 of them were selected as candidate SNP loci. Finally, 13 of the four rat populations of Wistar, GK, BN and SD were verified, which can distinguish the four SNP markers of strains of rats and their genotypes in each strain.

　　Conclusion: Thirteen SNP markers that can distinguish Wistar, GK, BN and SD rats were screened and verified, and these SNP markers can quickly distinguish Wistar, GK, BN and SD rats.