动物造模 z-bio 2022-06-21 545

　　OBJECTIVE: To analyze the polymorphisms in the regulatory region of the PPARγ2 gene promoter in Wuzhishan miniature pigs.

　　Methods: Five pairs of primers were designed according to the 2200bp regulatory region of the porcine PPARγ2 promoter to detect the polymorphism of 57 Wuzhishan miniature pigs in a closed group. Predictive analysis of RNA secondary structure and CpG islands.

　　RESULTS: Nine single nucleotide polymorphisms (SNPs) loci were screened, which were: -1595T/C, -1534G/A, -1262C/A, -1220C/A, and -1017A/G ､-963A/G, ､-955G/A, ､-866A/G, ､-333G/A. None of the SNPs were located in the promoter region identified by the software, but the -333G/A mutation was located in the core promoter (-656~-23bp) ) region. The transcription factor binding site near the mutated SNP site will change, the minimum free energy of the RNA secondary structure before and after the mutation will change, and its structure will also change significantly. No CpG island was found in the target sequence.

　　Conclusion: The screened SNPs in the PPARγ2 promoter regulatory region of Wuzhishan miniature pigs may affect the expression and regulation of this gene, which lays a foundation for further research on its function.