动物造模 z-bio 2022-10-06 642

　　Objective: To analyze the effect of storage and transportation conditions of three enteroviruses MHV, Reo-3 and MNV biological samples in experimental animals and mice on the detection results of pathogenic nucleic acids.

　　Methods: MHV, Reo-3, and MNV RNA viruses were mixed with the contents of mouse cecum to prepare reference samples; the preservatives included RNA extraction reagent lysate (BufferAVL) and normal saline, and the storage temperature was 4°C and room temperature (22°C). ~25°C); extract nucleic acid from each sample at 1, 2, 3, 7, and 14 days of storage, and use real-time fluorescence quantitative PCR to detect changes in the amount of virus samples.

　　Results: The detection amount of nucleic acid samples in saline as a preservative was lower than that in RNA extraction reagent lysate group, and the detection amount decreased significantly; the detection amount of samples in BufferAVL group stored at 4 ℃ was better than that of samples stored at room temperature of 25 ℃; 4 The three virus reference samples in the ℃-buffer AVL group had more than 50% of the virus detected at the 3-day time point, and were still detected at the 7-day time point, but could not be detected at the 14-day time point.

　　Conclusion: The storage and transportation conditions of the three mouse RNA virus samples are preferably to use RNA extraction reagent lysate, and store and transport at low temperature. It is best to complete nucleic acid detection within 3 days.