动物造模 z-bio 2022-10-10 652

　　Objective: To construct the differentially expressed miRNA and mRNA expression profiles in Chinese hamster oral buccal carcinoma (OSCC), and to preliminarily predict the miRNA and mRNA associated with the occurrence and development of OSCC.

　　Methods: The miRNA and mRNA expression profiles were constructed by next-generation high-throughput sequencing technology, and the miRNAs and mRNAs related to the occurrence and development of OSCC were predicted by Gene Ontology functional enrichment and KEGG Pathway analysis.

　　Results: The differentially expressed miRNA and mRNA expression profiles in Chinese hamster oral and buccal mucosal carcinoma were successfully constructed. A total of 11 known miRNAs, 3 new miRNAs were found to be significantly differentially expressed, and 194 mRNAs were differentially expressed, and one miRNA could regulate multiple miRNAs. One mRNA, and multiple miRNAs target and regulate a certain mRNA.

　　Conclusion: The differentially expressed miRNAs in OSCC in this experiment form a complex regulatory network by regulating mRNA, and play an important role in the occurrence and development of oral cancer, providing a theoretical basis for the occurrence, pathogenesis, clinical treatment and prognosis of oral cancer.