动物造模,系统性红斑狼疮 z-bio 2022-11-17 331

　　Objective: To investigate the relationship between the gut microbiota structure and anti-dsDNA antibodies in TC mice with systemic lupus erythematosus (lupus for short).

　　Methods: The serum anti-dsDNA antibody of lupus TC mice was detected by ELISA, and then the feces of the mice in the dsDNA positive group and the negative group were collected respectively, and Illumina HiSeq2500 high-throughput sequencing was used to perform 16S sequencing of the stool samples, and the intestinal tract between the two groups was compared. Relationship between microbial flora structure and anti-dsDNA antibody levels.

　　Results: The results of antibody detection by ELISA and high-throughput sequencing of mouse feces showed that the species community diversity of TC mice in the dsDNA positive group was significantly lower than that in the dsDNA negative group. Chloroflexi (Chloroflexi); class-level Betaproteobacteria (β Proteobacteria), Deltaproteobacteria (δ Proteobacteria) and Ktedonobacteria (Cinetobacter); order-level Burkholderiales (Burkella), Desulfovibrionales (Desulfovibrionales) Order) and Ktedonobacterales (Fibreobacteria); at the family level Alcaligenaceae (Alcaligenaceae) and Desulfovibrionaceae (Desulfovibrionaceae); at the genus level Parasutterella (Parasutterella) and Desulfovibrio (Desulfovibrio) differences Significant (P<0.05).

　　Conclusion: The structure of gut microbiota in lupus TC mice is highly correlated with anti-dsDNA antibodies. This study provides a basis for further elucidating the relationship between gut microbiota and the pathogenesis of systemic lupus erythematosus.