动物造模 z-bio 2022-11-18 768

　　OBJECTIVE: To analyze the genetic differences between BALB/c mutant curly-haired mice and normal BALB/c mice using microsatellite DNA marker technology.

　　METHODS: Thirty-eight microsatellite DNA loci were selected combined with fluorescent PCR technology and STR scanning genotype to detect the genetic variation of BALB/c mutant curly mice, hairless mice and normal mice.

　　Results: 27 of the 38 microsatellite loci were the same between BALB/c mutant curly hair mice and normal mice, and 11 loci were different, and the mutation rate was 28.9% (11/38). ); 30 loci were identical between BABL/c mutant hairless mice and normal mice, and 8 loci were different, with a mutation rate of 21.1% (8/38); while BABL/c mutant curly hair There were also 12 differences between mice and hairless mice.

　　Conclusion: The mutation rate of BALB/c mutant curly mice is higher, and significantly higher than that of hairless mice, which proves that the curly mouse mutation and the hairless mouse mutation are two completely different mutant lines, which can be used for future research. and developed applications in BALB/c mutant curly mice provide reliable theoretical data.