动物造模 z-bio 2022-10-13 741

　　Objective: To improve the positioning technique of orotracheal intubation in experimental rats, improve the efficiency of endotracheal intubation and reduce complications.

　　Methods: A combination of clinical trocar and venous indwelling needle was used to design a self-made tracheal intubation positioning anti-reflux device. 60 adult male SD rats were randomly divided into two groups, A and B, with 40 rats in group A for the positioning effect test and 20 rats in group B for the anti-reflux test. Group A was further divided into group A1 using a self-designed positioning device, group A2 using the fog method, and group A3 using the cotton fiber method for positioning judgment. Group B was divided into two subgroups, B1 and B2, and bubble escape was used as a positioning observation method. Orotracheal intubation by light transmission method was used to observe and record the positioning time, the positioning effect in the expiratory phase, and the length of the countercurrent water column in the inspiratory phase. In group A1, further tracheotomy was performed to clarify the location under direct vision.

　　Result: During the exhalation cycle, more than 3 bubbles were observed continuously escaping from the bubble outlet. Positioning time: group A1 needed (1.75±1.02) breathing cycles, group A2 needed (3.30±0.95) breathing cycles, and group A3 needed (4.10±0.99) breathing cycles. There was no significant difference between the A2 and A3 groups (P>0.05). The time required for the clear positioning of the A1 group was significantly less than that of the A2 and A3 groups, and the difference was significant (P<0.01). The longest countercurrent water column length in group B1 was (3.23±0.53) cm, and the longest countercurrent water column length in group B2 was (8.48±1.01) cm.

　　Conclusion: The self-made experimental rat orotracheal intubation anti-reflux device has a simple structure and is easy to use, which can effectively improve the positioning efficiency of tracheal intubation, and has good experimental application value.