动物造模 z-bio 2022-07-07 537

　　Objective: To use PKH26 and Hoechst 33258 to trace the localization of Uncv hairless mouse iRhom2 and its mutant proteins in Vero cells.

　　Methods: The cell membrane was stained with PKH26, and the nucleus was stained with Hoechst 33258 dye, and observed under a laser confocal microscope.

　　RESULTS: The target protein was observed by laser confocal fluorescence microscope, and it was found that wild-type iRhom2 was distributed in the cytoplasm of cells, while iRhom2mut existed in both the cytoplasm and the nucleus.

　　Conclusion: The differences in subcellular localization suggest that the N-terminal deletion mutation of iRhom2 may affect its intracellular localization.