【Animal modeling】-Analysis of ammonia gas and heavy metal content in drinking water of cynomolgus monkeys

  Objective: To detect the ammonia concentration and heavy metal content in drinking water in the monkey house of cynomolgus monkey breeding base.

  Methods: The air in monkey houses of different monkey groups, different feeding methods and different time points was collected, and the concentration of ammonia gas was detected by Nessler reagent spectrophotometry. Water samples were collected from five different locations in the breeding base, including groundwater outlet, reservoir, monkey house terminal, and sewage pool. Inductively coupled plasma mass spectrometry (ICP-MS) was used to detect the content of heavy metals.

  Result: The ammonia concentration in the quarantine group monkey house (0.59 ± 0.03) mg/ m3 was significantly higher than that in the population (0.34 ± 0.03) mg/ m3 and the commodity group (0.27 ± 0.04) mg/ m3. The ammonia content of monkey houses in different feeding methods was as follows: quarantine cage (0.59 ± 0.03) mg/ m3 > population cage (0.48 ± 0.02) mg/ m3 > commercial group cage (0.30 ± 0.02) mg/ m3 > commercial group cage Breeding (0.25 ± 0.01) mg/ m3 > population captive (0.22 ± 0.02) mg/ m3 ? Quarantine group cage? Population cage obviously higher ammonia concentration than other breeding methods. Different monkey groups and different feeding methods have the highest ammonia content in monkey houses before cleaning in the morning. The iron content in the drinking water of the monkey house terminal connected by the iron water pipe is higher than the drinking water content standard.

  Conclusion: The ammonia concentration in the monkey house in the cynomolgus monkey breeding base of this investigation is lower than the national standard, and the iron content in the terminal of the monkey house connected by the iron water pipe exceeds the standard.