动物造模 z-bio 2022-04-24 622

　　Objective: To improve the extraction of cerebrospinal fluid from rats by negative pressure suction device, and to further improve the success rate of cerebrospinal fluid collection in rats.

　　Methods: The original device was improved by increasing the flow regulator to control the intensity of negative pressure and to limit the amount of cerebrospinal fluid extracted each time; 36 male SD rats were randomly divided into 3 groups, and the percutaneous cistern puncture method and the negative pressure suction device were used respectively. method? The improved negative pressure suction device method was used to extract cerebrospinal fluid, and the Kruskal-Wallis rank sum test was used to evaluate the differences between the three methods?

　　Results: The improved negative pressure suction device method can effectively reduce bleeding, and the quality of cerebrospinal fluid obtained is higher than that of the negative pressure suction device method (P < 0.01) and the percutaneous cisternal puncture method (P < 0.001), and the success rate of taking qualified samples 75%?

　　CONCLUSION: The improved negative pressure suction device is easy to manufacture, simple to operate, less damage, less bleeding, and can significantly improve the success rate of cerebrospinal fluid collection in rats?