动物造模,气滞血瘀证 z-bio 2023-04-12 840

　　OBJECTIVE: To study the changes of the whole gene expression profile in the tongue of rats with qi stagnation and blood stasis syndrome and the biological processes and pathways involved in differential genes by using gene chip technology, in order to provide scientific basis for the study of blood stasis syndrome related theories and drug treatment.

　　Methods: High-fat diet combined with chronic unpredictable stimulation was used to replicate the animal model of qi stagnation and blood stasis, and gene chip technology was used to analyze the changes of the whole gene expression profiles and the pathways involved in the tongue of normal and model rats.

　　Results: Compared with normal rats, the results showed that there were 277 differentially expressed genes, including 68 up-regulated genes and 209 down-regulated genes. The results of GO and Pathway analysis showed that Qi stagnation and blood stasis syndrome were related to inflammation, lipid metabolism, immune response and other biological processes, as well as the changes of 7 pathways including complement and coagulation cascade pathway, PPAR signaling pathway, and cytochrome P450 foreign body metabolism pathway.

　　Conclusion: CYP450 and differential genes in complement and coagulation cascade pathway and PPAR signaling pathway may be involved in the pathogenesis of blood stasis syndrome, and provide a basis for the research of blood stasis syndrome and related drugs.