动物造模 z-bio 2022-06-30 438

　　Objective: To use the nested PCR method to detect the infection of Helicobacter pylori in the gerbil in vivo by gastric juice, so as to establish a detection technology that can continuously monitor the infection of Helicobacter pylori in the gerbil.

　　METHODS: Helicobacter pylori was cultured in vitro and inoculated into gerbils. The normal PCR method was used to detect the gastric juice of gerbils in the 10th week after infection. The contents of the colon and the feces of the colon were detected; at the same time, the accuracy of the nested PCR method was compared and analyzed by the rapid urease test and the serum ELISA method. The above PCR products were all verified by sequencing.

　　Results: The detection of gastric juice by ordinary PCR method, the detection of gastric juice, gastric tissue, duodenal contents, colon feces, rapid urease test, and serum ELISA detection method by nested PCR method were 30%, 100%, and 100%, respectively. %, 90%, 10%, 100%, and 0%. Comparing the detection results of different detection methods, it was found that the gastric juice nested PCR, gastric tissue nested PCR and rapid urease test all had a detection rate of 100%, ordinary PCR method for detecting gastric juice, colon stool nested PCR method and serological ELISA detection The detection rate of the method is relatively low.

　　Conclusion: The gastric juice nested PCR detection method can be used for long-term detection of Helicobacter pylori in gerbils due to its high specificity, accuracy and sensitivity, especially for the prevention and treatment of Helicobacter pylori infection.