动物造模 z-bio 2022-06-30 646

　　OBJECTIVE: To understand the effects of anesthetics and stress response on animal physiology, and to provide useful experience for the standardization of animal welfare and compound toxicity detection methods.

　　METHODS: SPF SD rats were selected as experimental animals, and were divided into 24 groups according to 4 factors, gender (A), fasting time (B), anesthesia method (C) and blood collection method (D). Blood samples were collected from SD rats in each group to measure red blood cell count, hemoglobin level, white blood cell count and its classification.

　　Results: The primary and secondary order of the influence of each factor on the white blood cell count was D > C > A > B. The white blood cell count of each factor level was male rats > female rats, venous blood > arterial blood, chloral hydrate > pentobarbital Sodium Sodium > No anesthesia. The primary and secondary order of the influence of each factor on the leukocyte classification result was C > D=A=B, and the leukocyte classification result of each factor level was chloral hydrate > pentobarbital sodium = no anesthesia. The primary and secondary order of the influence of each factor on the results of red blood cell count and hemoglobin level is C > D=A=B, the red blood cell count and hemoglobin level of each factor level is pentobarbital sodium > chloral hydrate > no anesthesia, fasting time in each group There was no statistical difference in hematological parameters between the two groups.

　　Conclusion: Fasting has no effect on hematological indexes, but there are differences in gender, arterial and venous hematological indexes; the effect on white blood cell count and classification of chloral hydrate anesthesia is greater than that of pentobarbital sodium anesthesia, while the effect on red blood cell count and hemoglobin level is Sodium pentobarbital anesthesia is greater than chloral hydrate anesthesia, and the two anesthesia methods have their own advantages and disadvantages.