动物造模,基因敲除 z-bio 2022-03-15 574

　　OBJECTIVE: To observe the survival curve of Presenilin-1/Presenilin-2 double knockout mice (dKO mice), and to provide a reference for further reproduction and the use of this model for the study of Alzheimer's disease (AD).

　　Methods: 50 female dKO mice, 30 males, and 20 females and 20 males of wild-type homologous mice were divided into 4 groups. The survival and lifespan of the mice in the 4 groups within 2 years under normal feeding conditions were observed. Plot survival curves for survival analysis.

　　Results: With the prolongation of time, the survival probability of female and male mice in dKO mice was lower than that of wild-type female and male mice, respectively, and the differences were statistically significant (P<0.001, P<0.001; P<0.001; P<0.001); There was no statistically significant difference in survival probability between female and male dKO mice.

　　CONCLUSION: Conditional knockout of presenilin-1 and presenilin-2 genes may shorten the lifespan of mice, and the survival probability is significantly lower than that of wild-type mice, while there is no statistical difference between female and male dKO mice. . The experimental results can provide a useful reference for the application of experimental design with the help of this model.