动物造模,脾脏间充质干细胞 z-bio 2022-03-15 468

　　Objective: To establish an efficient and reliable strategy for the isolation and expansion of mouse spleen mesenchymal stem cells by tissue block culture method.

　　Methods: The spleen tissue of mice was fully crushed under aseptic conditions, and the spleen matrix tissue obtained by digestion with collagenase was used to inoculate the digested tissue blocks in culture flasks. Their phenotypes were analyzed by cytometry, and their osteogenic and adipogenic differentiation was induced.

　　Results: A large number of spindle-shaped fibroid cells migrated and migrated from the digested spleen tissue block. The results of flow cytometry showed that these cells highly expressed CD29, CD44, Sca-1 and CD105, and lowly expressed CD11b, CD34, CD45 and Ia. The results of multi-directional differentiation experiments showed that the cells migrated from the spleen stromal tissue of mice had better adipogenic and osteogenic differentiation abilities.

　　Conclusion: The tissue block method can separate and culture mouse spleen mesenchymal stem cells. The obtained cells have high purity and strong differentiation ability, which provides a good cell model for related research.