动物造模 z-bio 2022-03-15 463

　　Objective: To investigate the infection of Helicobacter pylori and "Helicobacter rhesus monkeys" in domestic experimental rhesus monkeys and cynomolgus monkeys.

　　Methods: The primer sequences of Helicobacter 16S rRNA and Helicobacter pylori 16S rRNA from the reference, and the newly designed "H. rhesus" 16S rRNA-specific primers were used in 45 adult rhesus monkeys and 90 adult rhesus monkeys in captivity. In fecal samples of cynomolgus monkeys, the infection of the two types of Helicobacter in these two macaques was initially investigated by qPCR or conventional PCR detection.

　　RESULTS: The infection rates of Helicobacter pylori and Helicobacter rhesus in rhesus monkeys were both 100%, and the infection rates of Helicobacter pylori and Helicobacter rhesus in cynomolgus monkeys were 100% and 97.8%, respectively.

　　Conclusion: It is confirmed that the rhesus monkeys and cynomolgus monkeys artificially bred in my country are commonly infected with Helicobacter rhesus macaques. Helicobacter pylori and "Helicobacter rhesus monkeys" exist almost simultaneously in all artificially bred experimental monkey individuals, which may adversely affect the health of these two types of rhesus monkeys and the accuracy of related animal experimental results.