动物造模 z-bio 2022-11-16 860

　　Objective: To investigate the interference of Helicobacter hepatica infection on the surface molecular morphology and immune response of mouse bone marrow-derived dendritic cells (DC).

　　Methods: SPF grade BALB/c male mice were fed with H.hepaticus (ATCC 51450), and bone marrow DCs were isolated and cultured in vitro 5 months after the last inoculation. Interleukin-4 (IL-4) stimulates DC proliferation and differentiation, and the expression rates of DC cell surface molecules CD11c, CD40, CD80 and MHCⅡ were analyzed by flow cytometry. On this basis, mice in experimental group and control group were artificially inoculated with Newcastle disease virus (NDV) ZJ1 strain, and serum antibody titers of NDV were measured weekly to compare the differences in antibody production.

　　RESULTS: The expression rates of MHC II and CD40 molecules in the experimental group were higher than those in the control group. The level of NDV antibody in the experimental group was slightly lower than that in the control group in the first week; the antibody level of the mice in the experimental group was higher than that in the control group in the 2nd to 5th week, and the difference was significant; the serum antibody of the mice in the two groups decreased in the 6th week trend, the difference was not significant.

　　Conclusion: H.hepaticus infection can promote the maturation of mouse bone marrow DCs, increase the expression levels of MHC Ⅱ and CD40, and promote the production of anti-NDV antibodies in BALB/c mice.