动物造模 z-bio 2022-10-26 276

　　OBJECTIVE: To establish a mouse swimming endurance experimental system, explore new indicators for evaluating the swimming endurance of mice, and use this system to verify the effect of Rhodiola rosea on the endurance of mice.

　　Methods: The computer video acquisition technology and image analysis technology were applied to the mouse swimming endurance experiment, and the swimming information of the mice was obtained in real time, including the exhaustion time, the first sinking time, the total sinking time, and the first continuous sinking time. Based on multiple endurance evaluation indicators, the mouse endurance data can be objectively and accurately analyzed online; the stability and reliability of the system are experimentally studied by using normal mice and Rhodiola rosea-administered mice.

　　Result: There is a high correlation between computer recorded data and manual recorded data, r>0.95, the system is stable and reliable. The correlation coefficient between the first continuous sinking time and the exhaustion time was r=0.974, and the correlation coefficient between the other new indicators and the exhaustion time was r<0.9. Compared with the blank group, the Rhodiola rosea administration group had a significant increase in the first sinking time and the first continuous sinking time.

　　Conclusion: The indicators used to evaluate the swimming endurance of mice are objective, accurate and sensitive. The system realizes the integration of video acquisition, information integration, data analysis and statistical output, and finds a new indicator that is highly correlated with the time to exhaustion. sink time. The complete system is highly automated and intelligent, providing a reliable experimental system for the screening of anti-fatigue drugs.