动物造模,黑色素瘤 z-bio 2022-03-16 463

　　Objective: To screen the expression of protein 4.1 family in mouse melanoma cells, and to explore the effect of protein 4.1 on the proliferation of mouse melanoma cells.

　　Methods: Using genomic RNA and total protein of MEF, B16, B16-F10 cells as templates, the expression of protein 4.1 in mouse melanoma cells was screened by PCR and Western blot; eukaryotic expression vector pEGFP-N1- EPB41L3 was transfected into melanoma cells; the proliferation of B16 and B16-F10 cells was detected after expressing protein 4.1B.

　　RESULTS: The expression of protein 4.1B was lost in melanoma cells; the eukaryotic expression vector pEGFP-N1-EPB41L3 was successfully constructed by transfection, and it was found that the proliferation of melanoma cells expressing protein 4.1B was significantly inhibited.

　　Conclusion: The eukaryotic expression vector pEGFP-N1-EPB41L3 transfected to express protein 4.1B can significantly inhibit the proliferation of B16 and B16-F10 melanoma cells.