Objective: To understand the testing ability of laboratory animal testing institutions and improve the quality testing level of laboratory animals through the proficiency testing plan for rabbit hemorrhagic virus antibody detection.
Methods: According to the proficiency testing scheme approved by CNAS, the samples were prepared by screening serum, which passed the stability and homogeneity tests and were used as proficiency testing samples. Using random numbers, send samples to participating units, and attach work instructions. Submit the inspection report and the copy of the original record within the specified time limit, and the result will be judged as a satisfactory result if the result is consistent with the sample pre-inspection result, and the unsatisfactory result will be judged if it is inconsistent or fails to submit the result.
Results: 20 laboratories from 14 provinces, municipalities and autonomous regions signed up to participate in this comparison experiment, and all reported the test results within the specified time. The test results of 17 laboratories were qualified or excellent, accounting for 85% of the participating laboratories. %. Among the 20 laboratories, 14 laboratories adopted the enzyme-linked immunosorbent assay (ELISA) method, and 6 laboratories adopted the hemagglutination inhibition assay (HAI) method.
Conclusion: The overall detection ability of rabbit hemorrhagic virus antibodies in laboratory animal testing institutions across the country is relatively high, and the implementation of the proficiency testing plan can reflect the laboratory's detection level.