动物造模 z-bio 2023-03-10 496

　　Objective: To establish a method for SNP typing of frozen embryos and sperm in mice, which can be used for rapid genetic identification of frozen embryos and sperm.

　　Methods: Using frozen mouse embryos and sperm provided by the Shanghai Laboratory Animal Center of the Chinese Academy of Sciences (Shanghai Branch of the National Rodent Experimental Animal Seed Center) as samples, whole-genome amplification technology and PCR-LDR typing technology were used to establish mouse frozen SNPs Genetic identification methods.

　　Results: Whole-genome amplification technology can greatly increase the total amount of DNA in frozen embryo samples; PCR-LDR typing method is suitable for the typing of 45 SNPs in the whole mouse genome; C57BL/6, BALB/c, FVB /NJ and other embryos and 10 inbred lines for sperm, the SNP site information is consistent with the sequencing results; the number of frozen embryos in mice is proportional to the number of detected SNPs, and the detection rate of SNPs is 100% when the number of embryos reaches more than 12 .

　　Conclusion: Rapid SNP genotyping and genetic quality identification of inbred mouse embryos and sperm can be achieved.