动物造模,呼吸综合征 z-bio 2022-10-22 946

　　Objective: To screen minipig strains susceptible to highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) for the evaluation of HP-PRRS live vaccine.

　　Methods: In this study, Lantang pigs, Bama miniature pigs, Bama miniature pigs, and Wuzhishan miniature pigs (white line) were selected to inoculate PRRSV NVDC-JXA1 strain, and the local binary hybrid pigs were used as controls. After the challenge, the animals' spirit, appetite, and death were observed every day. The dead animals were necropsied, and the lung tissue was taken for RT-PCR detection of the virus. The observation was continued for 5 weeks.

　　RESULTS: After challenge, the animals in each group had symptoms such as depression, loss of appetite, and death. RT-PCR detection of dead animals was positive, and PRRSV antibody detection of surviving animals was positive. From the perspective of mortality, the white line of Bama miniature pig and Wuzhishan miniature pig had the highest sensitivity to PRRSV NVDC-JXA1 strain, and the sensitivity was higher than that of the control group.

　　Conclusion: The white lines of Bama minipigs and Wuzhishan minipigs are sensitive to HP-PRRSV and can be used for the test of HP-PRRS live vaccine.