动物造模 z-bio 2022-10-26 703

　　OBJECTIVE: To determine the growth and development characteristics of the self-established transgenic carcinogenicity model C57-ras mice and BALB/c mice for one generation, and to provide basic data for the commercial supply of this model.

　　Methods: Male transgenic positive C57-ras mice were mated with wild-type female BALB/c mice to obtain one generation of crosses (CB6F1 c-Ha-ras+/-), and the average litter size, weaning rate, and growth of positive mice were determined. Developmental status, transgene positive rate and other parameters.

　　Result: In terms of growth and development, the average number of females per litter was 8, the weaning rate was 90%, and the male-to-female ratio was in line with expectations. The mean birth weight of CB6F1 was 1.73±0.05g, and the mean weight of positive growing mice at 10 weeks of age was 24.38±1.74g for females and 29.42±1.72g for males. The body weight of males of growing mice was significantly higher than that of females, and there was a very significant difference (P<0.01); the positive rate of genotype detection was 46.9%, which was in line with the law of inheritance.

　　Conclusion: Transgenic carcinogenicity model C57-ras mice and BALB/c mice have normal growth and development characteristics in the first generation, which can realize large-scale commercial supply.