动物造模 z-bio 2022-05-31 600

　　Objective To investigate the effects of Oct4 and Cdx2 knockdown on the expression of genes related to the differentiation of bovine ICM and TE, and to explain the roles of Oct4 and Cdx2 in the early development of bovine embryos.

　　Methods RNA interference knocked down the Oct4 or Cdx2 gene in bovine embryos. Real-time quantitative PCR and immunofluorescence staining were used to detect the effects of knockdown of Oct4 or Cdx2 on the early development of bovine embryos and the expression of related genes.

　　Results The specific siRNA against Oct4 or Cdx2 gene could inhibit the expression of Oct4 or Cdx2 in bovine embryos. Oct4 knockdown had no effect on embryonic cleavage rate and 8-cell rate, but the blastocyst rate and mRNA level decreased significantly (P<0.05). Cdx2 knockdown inhibited Oct4 mRNA expression (P<0.05).

　　Conclusion Oct4 controls the differentiation of bovine embryos by regulating the expression of Cdx2, Sox2 and Nanog.