动物造模 z-bio 2022-03-18 377

　　Objective To explore the effect and mechanism of Shenqu Xiaoshi Oral Liquid on gastrointestinal motility in functional dyspepsia (FD) mice.

　　Methods 40 KM mice were randomly divided into normal group, Shenqu Xiaoshi oral liquid low-dose, medium-dose and high-dose groups, and the blood routine and liver and kidney function indexes of mice were detected. Another 50 KM mice were randomly selected as normal group, and the remaining mice were used irregular feeding + L-arginine (L-Arg) method to construct FD animal model, and then randomly divided into model group, Shenqu Xiaoshi orally. low-, medium- and high-dose groups; the body weight of the mice was recorded, the gastric residual rate and intestinal propulsion rate of the mice were calculated, and the pathological changes of gastric histopathology were observed. ) to detect the expression of endoplasmic reticulum stress factor inositol-requiring enzyme 1 (IRE1) and tumor necrosis factor receptor-associated factor 2 (TRAF2) in mouse gastric tissue.

　　Results White blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), platelets (PLT), lymphocytes (LYM), monocytes (MONO), neutrophils (NEU), aspartate Compared with aminotransferase (AST), alanine aminotransferase (ALT), total bile acid (TBA), blood urea nitrogen (BUN) and creatinine (CRE), there was no significant difference between groups (P> 0.05). ;The mucosal layer, submucosa, muscular layer and serosa layer of gastric tissue of mice in each group had clear structures, and no obvious pathological changes were found; Empty rate and intestinal propulsion rate were significantly increased, and the mRNA and protein expressions of IRE1 and TRAF2 in gastric tissue were significantly decreased (P<0.05).

　　Conclusion Shenqu Xiaoshi Oral Liquid has no significant effect on blood routine and liver and kidney function in normal mice, but can significantly improve gastrointestinal motility function in FD mice, which may be related to the down-regulation of the expressions of endoplasmic reticulum factors IRE1 and TRAF2.