动物造模,基因敲除,黑色素瘤 z-bio 2023-01-13 566

　　Objective: To investigate the effect of atrial natriuretic peptide on pulmonary metastasis of melanoma.

　　Methods: The melanoma B16F10 cell line cultured in vitro was injected into atrial natriuretic peptide knockout mice (ANP - / - mice) and C57BL/6J mice through the tail vein to establish a melanoma lung metastasis model. On the 21st day, the number of nodules transferred to the lung surface was counted, and the lung tissue was embedded, fixed, sliced, and stained with HE to determine and count the nodules metastatic to the lung.

　　Results: The number of melanoma nodules metastasized on the lung surface of ANP knockout mice was significantly less than that of C57BL/6J mice, and the difference was significant; the number of lung micrometastases in ANP knockout mice was also significantly less than that of C57BL/6J mice , the difference is significant.

　　Conclusion: ANP knockout significantly inhibited lung metastasis of melanoma.