动物造模 z-bio 2022-03-21 610

　　Objective To establish a rat astrocyte aging model, simulate the aging of astrocytes in aged rats, and explore the effect of aging astrocyte conditioned medium on the proliferation of neural stem cells.

　　Methods The neonatal rat cortical astrocytes and 15-day fetal telencephalic neural stem cells were isolated by trypsin digestion, and 200 μmol/L H2O2 was used to induce the astrocytes for 4 h to establish an aging model. On 7 days, the supernatant of senescent cells was used as the conditioned medium, and the ratio of 1:3 or 1:2 was added to the neural stem cell proliferation medium. Conditioned medium served as a control.

　　Results The 3-day normal conditioned medium had no effect on the short-term proliferation of neural stem cells, but had an inhibitory effect on the long-term proliferation; the 3-day aging conditioned medium inhibited the proliferation of neural stem cells; the 7-day normal conditioned medium promoted the short-term proliferation of neural stem cells, but had a positive effect on the long-term proliferation. Inhibitory effect; 7-day senescence conditioned medium inhibited the proliferation of neural stem cells, and the inhibitory effect was greater than that of normal conditioned medium.

　　Conclusion H2O2-induced senescent astrocytes inhibit the proliferation of neural stem cells, and the inhibitory effect on long-term proliferation is greater than that of normal astrocytes.