动物造模 z-bio 2022-03-21 480

　　Objective: To observe the effect of Xingnaojing injection on the dose-response relationship of Beagle dog coma.

　　METHODS: Forty-two male Beagle dogs, weighing 8-10 kg, were randomly divided into normal control group, model control group, Xingnaojing injection dose group 1 (0.43mL/kg) and dose group 2 (0.86mL/kg) according to body weight. , Dose 3 double administration group ((0.43+0.43) mL/kg), Dose 4 group (1.29 mL/kg), Dose 5 double administration group ((0.65+0.65) mL/kg), Mianning (0.02mL/kg) was anesthetized and fixed in a self-made striker. The striker was placed at 150 cm to strike the craniocerebral occiput of the dog vertically for 4 times to establish a coma model of traumatic brain injury in dogs. Immediately after modeling, different doses of Xingnaojing injection were administered, and the administration volume was 10.0 mL/kg, and the recovery time was observed, followed by brain MRI examination. Function, serum S-100 calcium binding protein (S-100B), neuron-specific enolase (NSE) content. The heart, liver, spleen, lung, kidney, adrenal gland and brain of each group were taken for histopathological examination.

　　RESULTS: Xingnaojing injection dose 1, dose 2 (single administration) and dose 3 (2 administrations) could significantly reduce the recovery time of dogs. Xingnaojing dose 1, Xingnaojing dose 2, Xingnaojing dose 4 can significantly reduce the content of serum S100B and NSE. Xingnaojing dose 1, dose 2, dose 3 (two doses), dose 4, and dose 5 (two doses) can significantly increase the area of injury recovery detected by canine brain MRI (P<0.05). Xingnaojing injection doses 1 and 2 can significantly reduce the number of lymphocytes and eosinophils in blood routine; Xingnaojing injection dose 1 can significantly reduce the number of mononuclear cells in dogs; Xingnaojing injection doses 4 and 5 There was no significant effect on the blood routine of dogs. There was no significant difference in liver and kidney function indexes (ALT, AST, TBil, BUN, CRE) between each administration group of Xingnaojing injection. There were no significant differences in the histopathology of the heart, liver, spleen, lung, kidney, and brain organs in each group.

　　Conclusion: Xingnaojing injection can significantly improve the degree of coma and brain injury caused by traumatic brain injury in Beagle dogs, and there is no obvious toxic and side effects when used in large doses.