动物造模 z-bio 2022-03-21 311

　　OBJECTIVE: To investigate the method of electrical stimulation of the cavernosal nerve of the penis to detect the intracavernous pressure, and to compare the differences between the two catheter materials (PE-50 tube needle and intravenous infusion needle) for detecting the intracavernous pressure of the penis.

　　METHODS: Thirty-six SPF male Sprague Dawley rats were selected, 16 were randomly selected as the control group, and the remaining 20 were in the experimental group, intraperitoneally injected with streptozotocin 60 mg/kg, and 16 type I rats were screened out after 8 weeks of feeding. Diabetic rats. The control group and the diabetes group were divided into the PE-50 cannula needle and the intravenous infusion needle group to record ICP with 8 rats each. Masson's trichrome staining and apomorphine experiments confirmed that the diabetic ED rat model was successfully established. After confirming the successful establishment of the model, the intracavernosal pressure was detected, and the related erectile function data recorded by PE-50 needle and intravenous infusion needle were compared.

　　Results: Masson's trichrome staining showed that compared with the control group, the diabetic model group decreased the area of corpus cavernosum smooth muscle, increased the area of collagen, and decreased the area of corpus cavernosum smooth muscle/collagen area, and the differences were statistically significant (P<0.05). The number of erections in the diabetes model group was significantly lower than that in the control group (P<0.01). the="" peak="" icp="" and="" mean="" arterial="" area="" under="" p="">0.05) recorded by pe-50 needle and intravenous infusion needle in normal group and diabetic group, while the peak rate (slope) was statistically significant (P<0.05 or P<0.01).

　　Conclusion: When the intracavernous pressure is recorded by electrical stimulation of the cavernosal nerve, the PE-50 needle and the intravenous infusion needle are both suitable for the measurement of intracavernous pressure.