动物造模,诱导糖尿病 z-bio 2022-03-21 429

　　Objective: To explore the effect of polysaccharides from the mushroom on the hypoglycemic effect of streptozotocin-induced type 1 diabetes (DM) rats.

　　METHODS: Forty male Wistar rats were selected, and 30 of them were injected intraperitoneally with 50 mg/kg STZ to establish a diabetic rat model. The successfully modeled rats were randomly divided into the polysaccharide group, model group and positive drug group. Glucose tolerance was measured after 60 days of intervention in each group. Serum was taken the next day to detect insulin, blood urea nitrogen, serum creatinine, triglyceride, and total cholesterol. The liver and kidney were taken out and weighed, and the supernatant superoxide dismutase and malondialdehyde content were detected after homogenization. Hematoxylin-eosin staining (HE staining) was used to observe the pathological changes of kidney and liver.

　　RESULTS: Compared with the model group, the polysaccharide group of C. japonicus could reduce the blood sugar, increase the body weight, increase the serum insulin, decrease the contents of blood urea nitrogen, serum creatinine, triglyceride and total cholesterol, and increase the SOD activity in the diabetic rats. MDA decreased, and there was a significant difference (P<0.01). The glomeruli were regular in shape, clear in structure, and reduced in vacuoles in hepatocytes.

　　Conclusion: The polysaccharide of the mushroom has a good antioxidant activity on STZ-induced diabetic rats, and plays a role in lowering blood sugar.