动物造模 z-bio 2022-03-21 859

　　Objective: To preliminarily explore the effect of high Axl expression on subcutaneous tumorigenesis of gastric cancer cells in nude mice.

　　Methods: The relationship between Axl and gastric cancer was analyzed by surgical pathological staining of gastric cancer; gastric cancer cells with high expression of Axl were constructed by transfecting lentivirus; gastric cancer cells were subcutaneously injected into nude mice to establish a subcutaneous tumorigenic model of gastric cancer cells; Subcutaneous tumor size was assessed in vivo.

　　RESULTS: Immunohistochemical results of the resected tissues of gastric cancer patients showed that the expression of Axl in gastric cancer tissue was significantly increased; the expression of Axl in the constructed gastric cancer cells with high Axl expression increased by 2.4 times. The high expression of Axl gastric cancer cells were used to establish subcutaneous tissue in nude mice. In the tumorigenic model, the in vivo imaging of small animals showed that after subcutaneous injection of cells for 2 weeks, the subcutaneous tumor formed by the high-expressing Axl cells was significantly larger than that of the control group, and the difference was more significant after 4 weeks (P<0.01). Finally, the high-expressing Axl gastric cancer cells Both the volume and weight of the formed subcutaneous tumors were significantly larger than those of the control group.

　　Conclusion: Gastric cancer tissues highly express Axl, and high Axl expression can enhance the subcutaneous tumorigenic ability of gastric cancer cell SGC-7901.