动物造模 z-bio 2022-09-23 334

　　Objective: To study the protective effect of trigonelline on heat stress-induced testicular injury in mice.

　　Methods: 120 male ICR mice were randomly divided into normal control group, model group and trigonelline drug intervention group (25mg/kg, 50mg/kg, 100mg/kg), 24 in each group. Mice in each group were given corresponding After one week of drug gavage, except for the normal control group, the lower abdomens of the other groups of mice were placed in a 43 ℃ constant temperature water bath for heat stress treatment for 15 min, and then the drug intervention group was given the corresponding dose of trigonelline by gavage for 14 d. The normal control group and the model group were given normal saline by gavage for 14 days, and the samples were taken at 1, 7, 10, and 14 days after the heat stimulation treatment. The reproductive organ index, sperm count, and HE staining were used to observe the structural changes of the testis, and then compared and analyzed.

　　Results: Compared with the normal control group, the reproductive organ coefficients and epididymal sperm counts of the mice in the model group were significantly decreased (P<0.05). The genital organ coefficients and epididymal sperm counts of mice in both groups were increased (P<0.05); histopathology showed that each dose of drug intervention group could significantly improve the heat stress-induced testicular injury in mice.

　　Conclusion: Trigonelline can significantly improve the degree of damage to the testis of mice caused by heat stress, and has a certain protective effect.