动物造模 z-bio 2022-07-07 247

　　OBJECTIVE: To investigate the effect of curcumin pretreatment on the changes of blood and tissue neutrophils in rats with heat stroke in desert dry heat environment.

　　Methods: 160 SPF male SD rats were selected and randomly divided into 4 groups according to the curcumin gavage dose. After continuous gavage of equal volume in each group for 1 week, the rats were placed in an artificial experimental chamber in a special environment in Northwest China, and a desert was set up. In the dry heat environment climate model, at the time points of 0min, 50min, 100min and 150min, 10 rats in each group were randomly selected out of the cabin for anesthesia, and the number of blood neutrophils was measured; at the time point of 150min, the ileum, liver and kidney of each group were collected. , Lung tissue, HE staining, observation under microscope, the number of infiltrated neutrophils and histological lesions were recorded.

　　Results: The blood neutrophils in each group showed an upward trend from 0 min to 50 min; the blood neutrophils in each group showed a downward trend from 50 min to 150 min. The number of neutrophil infiltration in the ileum, liver, kidney and lung at 150 min increased with the With the increase of curcumin pretreatment concentration, all showed a downward trend, and the number of neutrophils in the medium and high concentration groups was significantly lower than that in the low concentration group and the saline group (P<0.01). Sexual analysis showed that the number of blood neutrophils was significantly negatively correlated with the number of neutrophil infiltration in tissues.

　　Conclusion: Under the continuous exposure of dry heat environment, the number of neutrophils in the blood of rats increased at first and then decreased gradually; with the aggravation of tissue damage, the number of neutrophils infiltrated in tissues increased, and the number of neutrophils in blood increased. The decrease of cells is closely related to the increase of tissue neutrophil infiltrating cells. Curcumin may alleviate tissue damage by inhibiting the decline of blood neutrophils and reducing tissue neutrophil infiltration in heatstroke rats.