动物造模,小鼠肺纤维化 z-bio 2022-09-23 424

　　Objective: To inject bleomycin into C57BL/6J mice to induce pulmonary fibrosis by three different methods: lumbar puncture tracheal intubation, indwelling needle tracheal intubation and tracheotomy, and to explore the modeling of lumbar puncture tracheal intubation The effects of three methods, namely, indwelling needle tracheal intubation modeling method and tracheotomy modeling method on mice.

　　METHODS: 120 C57BL/6J mice were randomly divided into blank group, lumbar puncture tracheal intubation model group, indwelling needle tracheal intubation model group and tracheotomy model group. The general condition (body weight change and survival rate), histopathological changes (HE and Masson staining) and hydroxyproline levels of the mice were observed.

　　Results: The body weight of mice in lumbar puncture tracheal intubation model group and indwelling needle tracheal intubation model group were higher than those in tracheotomy model group 28 days after modeling, with statistical difference (P<0.01, P<0.001); There were differences in the survival rate of mice. Except for the blank group, the tracheotomy model group was the lowest, the indwelling needle tracheal intubation model group was the highest, and there was a statistical difference between the blank group and the tracheotomy model (P<0.05). There was no statistical significance; the degrees of alveolitis and pulmonary fibrosis in the lung tissue were tracheotomy model group, lumbar puncture tracheal intubation model group and indwelling needle tracheal intubation model group from high to low, and the most severe 21 days after modeling. ; Lesions in the tracheotomy model group were evenly distributed, while the lumbar puncture tracheal intubation model group and the indwelling needle tracheal intubation model group were unevenly distributed, and the lung tissue around the trachea was heavier; the lumbar puncture tracheal intubation model group and the tracheotomy model group were not evenly distributed. The level of hydroxyproline in the model group at 28 days after modeling was significantly higher than that in the blank group (P<0.01, P<0.001).

　　Conclusion: Considering a variety of factors, the lumbar puncture and tracheal intubation modeling method is the best method among the three modeling methods.