动物造模,尿毒症 z-bio 2022-03-22 458

　　Objective: To compare the application of two hemostatic methods in rat uremic model.

　　METHODS: Male SD rats were divided into two groups, A and B. The uremia model was established by 2/3 left kidney resection and right nephrectomy. In group A, 3M tissue glue was used to stop bleeding during the resection of left kidney 2/3, and in group B, electrosurgery was used. Coagulation and hemostasis were performed, intraoperative blood loss and left renal blood flow occlusion time were compared, and the survival rate of rats was compared after operation. Pathological examination.

　　RESULTS: The serum creatinine of the two hemostatic methods increased to meet the requirements of the uremic model; the left renal blood flow blocking time in group A was (59.97±7.56)s, and that in group B was (174.1±15.28)s (P<0.05); A The bleeding volume of left nephrectomy was (0.11±0.037) mL in group B and (0.24±0.056) mL in group B (P<0.05); renal histopathology in both groups showed glomerular ischemic shrinkage, tubulointerstitial fiber The postoperative survival rate of group A was significantly higher than that of group B (P<0.05).

　　Conclusion: 3M tissue glue is superior to electrocoagulation for hemostasis in terms of bleeding time, bleeding volume and postoperative survival rate.