动物造模,肾间质纤维化 z-bio 2022-04-24 368

　　Objective: To investigate the intervention effect and mechanism of down-regulation of miR-192 on renal interstitial fibrosis in rats with unilateral ureteral obstruction (UUO) after renal injury

　　Methods: Forty-five male SD rats were randomly divided into sham operation group, model group, and antagomir-192 group. Except for the sham operation group, the other two groups of rats were all established renal interstitial fibrosis rat model by ligation of the left ureter. On the 1st, 7th, and 14th days, the rats in the antagomir-192 group were injected with antagomir-192 through the tail vein, while the rats in the sham-operated group and the model group were injected with the same volume of normal saline through the tail vein. PCR) to detect the expression of miR-192 in the renal tissue of the obstructed side. Blood was taken from the heart to determine the content of blood urea nitrogen (BUN) and creatinine (Cr) in the serum of the rats. HE staining was used to observe the morphological and pathological changes of the renal tissue of the obstructed side. Masson staining was used to calculate the interrenal of the obstructed side. Detection of epithelial-mesenchymal transition (EMT) related markers E-cadherin, α-smooth muscle actin (α-SMA) and vimentin in obstructed kidney tissue by Western blotting ) and the expression changes of collagen I (collagen I)

　　Results: Compared with the sham-operated group, the expression level of miR-192, BUN, Cr content in serum, renal interstitial injury score, and renal interstitial collagen fiber deposition rate were significantly increased in the model group ( P<0 01). The expression of E-cadherin protein in tissue was significantly decreased ( P<0 01), and the expression of α-SMA, vimentin and collagen I protein was significantly increased ( P<0 01). Compared with the model group, the antagomir-192 group had miR-192 in kidney tissue. The expression level of BUN and Cr in serum, renal interstitial injury score, and renal interstitial collagen fiber deposition rate were significantly decreased ( P < 0 01) E-cadherin protein expression in renal tissue was significantly increased ( P < 0 01) α- The protein expressions of SMA, vimentin and collagen I were significantly decreased ( P<0 01)

　　Conclusion: The expression of miR-192 is increased in renal tissue of renal interstitial fibrosis rats. Down-regulation of miR-192 can inhibit renal interstitial fibrosis in UUO rats and play a protective role.