动物造模,高血压 z-bio 2022-05-31 277

　　Objective: To study the effect of Astragalus on endoplasmic reticulum stress response (ERS) in vascular remodeling in hypertensive rats, and to explore the molecular mechanism of its vascular protection.

　　Methods: A total of 140 rats were divided into control group, model group and intervention group. A hypertensive rat model was established by abdominal aortic stenosis, and rats in the intervention group were intraperitoneally injected with Astragalus injection 8 g/(kg·d). At 1, 2, 4, and 6 weeks after the operation, the rat tail artery manometry was used to measure the blood pressure of the rats, the thickness of the vascular muscle layer, the expression of CRT and caspase-12 by Western blot, and the apoptosis of vascular smooth muscle cells (VSMC) by TUNEL method. death rate.

　　Results: In the model group, the morphology of VSMCs changed after operation, blood pressure, arterial wall muscle layer thickness and VSMC apoptosis rate increased in a time-dependent manner. However, the expression of caspase-12 molecule increased after 2 weeks, and the high expression became more significant with time delay. Compared with the model group, the astragalus intervention showed a certain improvement in VSMC morphology, blood pressure, vessel wall muscle layer thickness and VSMC apoptosis. At the same time, astragalus can inhibit the early high expression of CRT, and can inhibit the high expression of caspase-12, and the inhibitory effect is more obvious with the delay of time.

　　Conclusion: Astragalus has a certain antihypertensive effect on hypertensive rats and can improve vascular remodeling, and its mechanism may be related to its regulation of ERS protective and pro-apoptotic factors.