动物造模 z-bio 2022-03-28 334

　　Objective: To investigate the effects of different doses of perfluorooctane sulfonate exposure on embryonic development and placental insulin-like growth factor-1 expression in rats.

　　METHODS: Forty-eight SD female rats on the 12th day of pregnancy were randomly divided into 4 groups and given different doses of PFOS (0, 5, 10, 20 mg/kg). Body weight, fetal liver coefficient, serum PFOS and glucocorticoid (GC) levels of pregnant mice, and placental IGF-1 expression levels of mice and fetuses.

　　Results: Compared with the control group, with the increase of the dose of PFOS, the serum PFOS of the pregnant mice in the exposure group was significantly accumulated, and the body weight of the pregnant mice, the body weight and length of the fetal mice, and the weight of the placenta gradually decreased, and the difference in the PFOS 20 mg/kg group was significant. The liver coefficient of fetal rats also showed the same trend, the low, medium and high dose exposure groups were significantly lower than the control group (P<0.05), the blood biochemical alanine aminotransferase (ALT) and Tianmen Aspartate aminotransferase (AST) also increased with the increasing dose of PFOS (P<0.05); in PFOS 10 mg/kg group, the serum GC level of pregnant mice increased (P<0.05); placental IGF- 1 The expression level decreased with increasing PFOS dose.

　　Conclusion: Long-term exposure to PFOS during pregnancy can lead to the accumulation of serum PFOS in pregnant mice, which in turn leads to fetal liver toxicity, increased GC content, and decreased placental IGF-1 expression, which ultimately affects the growth and development of fetal mice.