动物造模,糖尿病 z-bio 2022-11-05 593

　　OBJECTIVE: To observe the effect of maca combined with exercise therapy on renal mitochondrial respiratory enzyme activity and renal function in diabetic rats.

　　Methods: 40 diabetic model rats were randomly divided into model control group, maca group, exercise group and maca+exercise group. Maca group and maca+exercise group were given daily maca powder (5g/kg) by gavage. , the exercise group and the maca + exercise group underwent treadmill training. After 6 weeks, the rats in each group performed exhaustive exercise (35 m/min), and then immediately anesthetized to extract kidney mitochondria, and the mitochondrial respiratory chain enzymes RCCI, RCCII, RCCIII, RCCIV were measured. Activity and blood urea nitrogen (BUN), serum creatinine (SCr), blood glucose (bloodglucose) content.

　　Results: Compared with the control group, the activities of renal mitochondrial respiratory chain enzyme RCCII in the maca group, RCCI, RCCII in the exercise group, and RCCI, RCCII and RCCIV in the maca + exercise group were significantly increased; compared with the maca group, the activities of maca + exercise Compared with the exercise group, the activity of RCCII in the maca+exercise group was significantly increased. Compared with the maca group, the serum creatinine and blood sugar levels of the maca+exercise group decreased, and the difference was significant (P<0.05 or P<0.01). Compared with the exercise group, the blood urea nitrogen and blood glucose levels of the maca + exercise group were decreased, and the difference was significant (P<0.05).

　　Conclusion: Maca combined with exercise therapy can improve renal mitochondrial respiratory function, increase the level of oxidative phosphorylation and improve renal function in aged diabetic rats.