动物造模 z-bio 2022-03-28 467

　　Objective: To investigate the effect of Xingnao Zaizao capsule on the memory impairment induced by formaldehyde in mice and its mechanism.

　　Methods: SPF grade KM mice were randomly divided into blank control group, solvent control group and low, medium and high dose groups of 5 groups, with 14 mice in each group. The mice were exposed to poison in a poisoning device for 14 days. The learning and memory ability of the mice in each group was detected by the dark avoidance experiment; the activities of nitric oxide synthase (NOS), superoxide dismutase (SOD) and Malondialdehyde (MDA) levels; hematoxylin-eosin staining was used to observe the number and morphological changes of pyramidal cells in the CA1 region of the mouse hippocampus.

　　Results: Compared with the blank control group, the dark avoidance latency of the solvent control group was shortened (P < 0.01), the number of mistakes was increased (P < 0.01), the activity of NOS and SOD in the brain tissue was decreased (P < 0.05), and the level of MDA was increased (P < 0.01), the number of pyramidal cells in the hippocampal CA1 area was reduced, the arrangement was sparse and disordered, and the shape was irregular. Compared with the solvent control group, the incubation period of the mice in the medium and high dose groups were prolonged (P < 0.05), and the NOS and SOD activities were significantly higher. increased (P < 0.01), the number of mistakes in the low, medium and high dose groups decreased (P < 0.05), and the level of MDA decreased (P < 0.01). Pyramidal cells in the hippocampal CA1 region of mice in the low and medium dose groups The number of layers increased slightly, and the pyramidal cells in the hippocampal CA1 region in the high-dose group were clearly layered, tightly arranged, and the number increased significantly.

　　Conclusion: Xingnao Zaizao Capsules can improve the memory impairment induced by formaldehyde in mice, and the mechanism may be related to its anti-oxidative damage effect and increasing the number of pyramidal cells in hippocampal CA1 area.