动物造模 z-bio 2023-03-08 534

　　OBJECTIVE: To study the distribution and expression of lysozyme LYZ1 gene in hatched blastocysts and dormant embryos of mice before and after superovulation, and to explore the new regulatory mechanism of animal embryo implantation.

　　Methods: Normal hatching blastocysts and superovulated blastocysts were obtained from pregnant 5dICR mice, and dormant embryos and superovulated dormant embryos were obtained on the 8th day of gestation using the mouse delayed implantation model. LYZ1 protein was detected by immunofluorescence and Western Blot methods. Distribution and differential expression changes in four groups of embryos.

　　Results: LYZ1 was expressed in the hatched blastocysts and dormant embryos of mice before and after superovulation, and was mainly concentrated in the inner cell mass, rarely distributed in trophoblast cells and cytoplasm. In contrast, the expression of LYZ1 protein was significantly up-regulated in mouse embryos after superovulation. Compared with mice without a dormant model, the expression of LYZ1 protein was significantly up-regulated in embryos of dormant model mice.

　　Conclusion: LYZ1 protein is expressed in the blastocyst inner cell mass and may be involved in regulating the development of embryonic inner cell mass; LYZ1 protein is highly expressed in superovulated-dormant embryos, indicating that LYZ1 protein is under the dual influence of dormancy and superovulation. Will rise for protection against unfavorable environment.