动物造模,肺损伤 z-bio 2022-03-28 685

　　Objective: To investigate the protective effect of mild hypothermia on radiation-induced lung injury, and to compare the protective effect with amifostine, a commonly used clinical anti-radiation drug.

　　METHODS: 75 male SD rats were randomly divided into blank control group, simple irradiation group, amifostine positive control group, mild hypothermia prevention group and mild hypothermia treatment group. Except blank control group, all the other groups received 20Gy electron beam full chest For a single irradiation, the amifostine positive group was given intraperitoneal injection of amifostine 30 min before irradiation; the rats in the mild hypothermia prevention group were irradiated after the body temperature dropped to a mild hypothermia state and maintained for 6 h; the rats in the mild hypothermia treatment group were irradiated Body temperature was kept at mild hypothermia for 6 hours. On the day, 14th and 35th day after irradiation, lung tissue histopathological examination and collagen fiber examination were performed to determine the content of malondialdehyde (MDA) in serum of rats, and the reduction of glutathione. The content of glutathione (GSH) and the activity of superoxide dismutase (SOD), the content of TNF-α in lung tissue homogenate, and the apoptosis of lung tissue were observed.

　　Results: After irradiation, the SOD activity of each irradiation group decreased immediately, and the SOD activity of the mild hypothermia treatment group was higher than that of the simple irradiation group on the 14th day (P<0.05). Compared with the simple irradiation group, the amifostine positive group and the mild hypothermia intervention group On the 14th day, the levels of MDA in serum were significantly decreased (P < 0.05); the levels of TNF-α in the simple irradiation group were significantly increased 6 h after irradiation, and the amifostine positive group and mild hypothermia treatment group had a higher degree of increase than the simple irradiation group. On the day of irradiation, the apoptotic cells in the simple irradiation group increased significantly and were widely distributed, and all the intervention groups decreased accordingly, but the difference between the groups was not significant.

　　Conclusion: Mild hypothermia treatment can improve the antioxidant capacity, anti-inflammatory capacity and anti-apoptosis capacity of radiation-induced lung injury rats, and the effect is similar to that of the commonly used clinical drug amifostine.