The latest advances in animal experimental pathology technology doubled the results in the medical field. In experimental pathology techniques, the selection and fixation of specimens and subsequent generation and staining have a significant impact on the reliability of pathological results. Some scientific researchers do not understand pathology methods, lack basic pathology knowledge, and draw wrong conclusions, causing casualties. A common feature is that they underestimate the pretreatment of pathological tissue specimens. This is a very important step in pathological research. Scholars from Urumqi General Hospital of Lanzhou Military Region have gathered years of experience in animal experimental pathology techniques, shared with readers some problems that should be paid attention to in the early processing of experimental pathological specimens, and made contributions to the continuous development of experimental animals.