动物造模 z-bio 2022-03-29 281

　　OBJECTIVE: To explore the establishment of a model of mouse emotional-cognitive behavioral damage caused by high-frequency electromagnetic radiation, and to provide an experimental basis for the study of radiation damage mechanism and protection.

　　Methods: The high-frequency electromagnetic reverberation chamber can generate continuous and uniform electromagnetic radiation waves. Mice were exposed to the high-frequency electromagnetic radiation box for 1d, 3d and 7d respectively, and a series of behavioral tests were carried out. behaviors, depression-like behaviors, and changes in cognitive function.

　　Results: High-frequency electromagnetic radiation (frequency 3GHz, SAR value 4W/kg) was exposed for 4 hours a day for 7 days, which did not affect the autonomous activities of mice, but it could lead to the number of times the mice in the radiation group entered the central area of the opening field (P ＜ 0). .05) and time (P ＜ 0.05) were significantly reduced; compared with the unexposed group, the mice in the irradiated group significantly decreased the number of times (P ＜ 0.05) and the percentage of time (P ＜ 0.05) and the percentage of time ( P < 0.01); at the same time, exposure to electromagnetic radiation at this dose also significantly increased the immobility time of mice in forced swimming and tail suspension experiments (P < 0.01, P < 0.05); The unexposed group of mice significantly reduced the discrimination index for novel objects in the novel object recognition experiment (P < 0.001).

　　Conclusion: Continuous high-frequency electromagnetic radiation (frequency is 3 GHz, SAR value is 4 W/kg) can time-dependently induce anxiety-like-depression-like behavior and cognitive impairment in mice, which provides an experiment for the study of related prevention and treatment techniques. in accordance with.