动物造模,自发性糖尿病 z-bio 2022-03-29 276

　　Objective: To investigate the effect of vitamin D (VD) deficiency on the pathogenesis of spontaneous diabetic rats.

　　Methods: Male 5-6 week old Zucker control mice and model mice (ZDF) were randomly divided into 4 groups according to body weight, namely normal control group (ZL), VD deficient control group (ZL + VD.Def) and model group (ZDF). And VD lacks model group (ZDF + VD. Def). The rats in each group were fed to 12 weeks of age, and the body weight, food intake, water intake, urine volume, urine sugar, and blood sugar were monitored during the feeding process, and oral glucose tolerance test was performed at 11 weeks of age; HE staining was used to observe the morphology and structure of islets in rats change.

　　Results: The body weight of the rats in the VD deficiency model group was significantly higher than that in the model group; the increase of water intake and urine volume were earlier than those in the model group; the blood glucose of the rats in the VD deficiency model group increased significantly earlier than that in the model group, and the VD deficiency model at each stage was significantly higher than that in the model group. The blood glucose in the VD deficiency group was significantly higher than that in the model group, about 2.0 times that in the model group at the age of 12 weeks; compared with the model group, the glucose tolerance of the rats in the VD deficiency model group was impaired. Islet cell damage is more serious.

　　Conclusion: VD deficiency accelerates and aggravates the onset of diabetes in ZDF rats, and VD deficiency is a key factor in the onset of obese T2DM.