动物造模 z-bio 2022-03-29 245

　　Objective: To observe the changes of dopamine (DA) content in the lateral geniculate body in guinea pig models of flicker-induced myopia and form deprivation myopia, and to conduct a comparative analysis, and to preliminarily explore the central pathogenesis of different myopia models.

　　Methods: Twenty-four ordinary 2-week-old guinea pigs were randomly divided into 3 groups (n = 8): stroboscopic light (FLM) group, form perception deprivation (FDM) group, and control group. Each group was raised for 8 weeks? The diopter and axial length of the right eye of the guinea pigs in each group were measured respectively. After the 8-week experiment, the DA in the lateral geniculate body of the left brain was quantified by high-performance liquid chromatography-electrochemical detection method (HPLC-ECD).

　　Results: Before modeling, there was no significant difference in the diopter and axial length of each group ( P > 0.05). At the 8th week of modeling, compared with the control group, the diopter changes of the right eye in the FLM group and the FDM group ( P < 0.001) There were significant differences in the changes in axial length (P<0.05), indicating="" that="" myopia="" modeling="" was="" hplc-ecd="" da="" flm="">control group> FDM group; control group was (37.04 ± 1.18) pg / μL; the FDM group was (24.27 ± 3.46) pg/ μL, which was significantly different from the control group (P = 0.021); the FLM group was (45.58 ± 1.98) pg/ μL, which was significantly different from the control group Sex (P=0.01)?

　　Conclusion: The DA content in the lateral geniculate body was increased in the stroboscopic light-induced myopia model, while the DA content in the form deprivation myopia model was decreased, indicating that the expression of DA in the lateral geniculate body of the two experimental myopia models was inconsistent. Could the mechanism be different?